The engineering of sucrose phosphorylase has emerged as a dynamic field that underpins sustainable production methods for value-added compounds derived from sucrose. By tailoring enzyme active sites, ...

A new technique to quantitate nucleoside phosphorylase (NP) activity in single or small numbers of counted visually selected cells is presented. Fibroblasts were cultivated on the plastic film bottom ...

Mammalian cells have enzymes to convert adenosine to inosine by deamination and inosine to hypoxanthine by phosphorolysis, but they do not possess the enzymes necessary to form the free base, adenine, ...